site stats

Cryaa promoter

WebThe mRNA and protein levels of CRYAA were significantly reduced in the lens epithelia of age-related nuclear cataract cases vs. age-matched controls, which corresponded to hypermethylation of the CpG island of CRYAA promoter. Treatment with a DNA-demethylating agent was associated with restoration of CRYAA expression. WebPromoter methylation of RASEF was associated with increased risk of death due to metastasis, ... Down-regulation and CpG island hypermethylation of CRYAA in age-related nuclear cataract. FASEB J 2012; 26: 4897-902. doi: 10.1096/fj.12-213702. 7. Zhu XJ, Zhou P, Zhang KK, Yang J, Luo Y, Lu Y, et al.

Differentiation of Induced Pluripotent Stem Cells to Lentoid Bodies ...

WebMar 21, 2024 · Size: 175 amino acids Molecular mass: 20159 Da Protein existence level: PE1 Quaternary structure: Heteromer composed of three CRYAA and one CRYAB subunits (PubMed:20836128). Aggregates with homologous proteins, including the small heat shock protein HSPB1, to form large heteromeric complexes (PubMed:10751411). Inter-subunit … WebDec 1, 2024 · Notably, the adenovirus E1b promoter, the zebrafish lepb 0.8-kb (P0.8) and lepb 2-kb (P2) promoters, and a new zebrafish synthetic promoter (ZSP) that combines elements of the E1b and P0.8 promoters drove little or no ectopic expression, making them suitable for transgenic assays. We also found significant differences in compatibility … newberg oregon burn information https://chriscrawfordrocks.com

Epigenetic Regulation of αA-crystallin in High Myopia-Induced …

WebSV40 polyA, zebrafish cryaa promoter (Kurita et al., 2003), AmCyan, SV40 polyA. For the myl7:Cre, cryaa:YPetulm13Tg line (abbreviated myl7:Cre), the following elements were assembled by PCR-based cloning techniques in a vector containing MiniTol2 repeats for transgenesis: 5.1 kb fragment of the zebrafish myl7 promoter, Cre recombinase WebApr 1, 2016 · The cryaa promoter drives high levels of expression in the lens of larval and adult zebrafish; when driving a fluorescent protein, it serves three purposes. First, it allows for the assessment of the efficiency with which the BAC transgene was injected into embryos based on the proportion of embryos with mosaic fluorescent protein expression … WebMar 17, 2016 · The CRYAA promoter with the rs7278468_T or rs7278468_G was transfected into HLE cells along with si-NC or si-KLF10, and the luciferase activity was measured 48 hours after transfection (the ... newberg or weather forecast

Plasmid Set for Efficient Bacterial Artificial Chromosome (BAC ...

Category:CpG site methylation in CRYAA promoter affect transcription …

Tags:Cryaa promoter

Cryaa promoter

Tol2 Pdest Cryaa Venus Polya Destination Vector utah medical

WebJun 20, 2016 · A Cryaa promoter-tdTomato plasmid was gifted by T. Xu (Yale) . The Cryaa promoter-tdTomato cassette was released by restriction with MluI and AflII and ligated in compatible sites between 5´and 3´ piggyBac ITR´s, resulting in pTTCryTom (cryTom) plasmid consisting of the alphaA-crystallin promoter, tdTomato cDNA and a SV40 poly … WebJun 5, 2015 · CRYAA transcript and protein levels are both downregulated, and the CpG island in the CRYAA promoter is hypermethylated in human age-related cataract (Zhou et al., Reference Zhou, Luo, Liu, Fan and Lu 2012). We performed promoter methylation analysis of six genes, including TRAM1, CRYAA, HSF4, VEGFA, GJA3, and FTL. …

Cryaa promoter

Did you know?

WebDec 3, 2013 · DNA methylation was analyzed by pyrosequencing the CpG islands in CRYAA promoter. CRYAA expression was compared among high-myopic eyes with … WebMar 17, 2016 · (a) The CRYAA promoter containing either the T-C-G or C-G-T haplotype was transfected into HLE cells and Luciferase activity was measured 72 hours after …

WebJun 20, 2016 · A Cryaa promoter-tdTomato plasmid was gifted by T. Xu (Yale) [6]. The Cryaa promoter-tdTo-mato cassette was released by restriction with MluI and AflII and ligated in compatible sites. WebMar 13, 2024 · We thus generated a transgenic line by placing a GFP coding region along with a β-globin leader sequence and SV-40 poly(A) signal under the control of a cryaa promoter region (Fig. 6A). We reasoned that this transgene should faithfully recapitulate the transcriptional activation of the endogenous gene, but the stability and polyadenylation of ...

WebDec 2, 2016 · Whereas in the mouse the γ-crystallin mutants were under the control of their respective native promoters, the use of the αA-crystallin (cryaa) promoter to drive the expression in the zebrafish lens may reduce the overall concentration of these proteins. This may allow the distinction of intrinsic differences in the aggregation propensity of ... WebApr 1, 2024 · All the participants were genotyped for two tag-SNPs in TLR4 (rs1927914 in the promoter region and rs11536889 in the 3′-untranslated region) using the KASP method. ... CpG site methylation in CRYAA promoter affect transcription factor Sp1 binding in human lens epithelial cells. BMC Ophthalmol. (2016) T. Li et al.

Web035233 STOCK Tg(Cryaa-cre)10Mlr/J MLR10 transgenic mice exhibit cre recombinase expression in both the lens epithelium and lens fibers under the transcriptional control of the mouse Cryaa (crystallin, alpha A) promoter modified by the insertion of a Pax6 consensus binding element/lens epithelial cell enhancer. Cre expression is not found in other parts …

WebPolymorphisms in alpha A crystallin (CRYAA) gene have been implicated in susceptibility to cataracts, but some published studies have reported inconclusive results. Our study … newberg oregon chevy dealerWebFeb 22, 2024 · The fact that both GJA8 and CRYAA function to stimulate visual development and are required during retinal development in mice and humans suggests … newberg oregon 55+ communitiesWebFeb 22, 2024 · 66. ]. The fact that both GJA8 and CRYAA function to stimulate visual development and are required during retinal development in mice and humans suggests that GJA8 and CRYAA are key genes for the promotion of retinal development and alleviation of the symptoms of visual impairments in mammals. newberg oregon bed and breakfast