WebThe mRNA and protein levels of CRYAA were significantly reduced in the lens epithelia of age-related nuclear cataract cases vs. age-matched controls, which corresponded to hypermethylation of the CpG island of CRYAA promoter. Treatment with a DNA-demethylating agent was associated with restoration of CRYAA expression. WebPromoter methylation of RASEF was associated with increased risk of death due to metastasis, ... Down-regulation and CpG island hypermethylation of CRYAA in age-related nuclear cataract. FASEB J 2012; 26: 4897-902. doi: 10.1096/fj.12-213702. 7. Zhu XJ, Zhou P, Zhang KK, Yang J, Luo Y, Lu Y, et al.
Differentiation of Induced Pluripotent Stem Cells to Lentoid Bodies ...
WebMar 21, 2024 · Size: 175 amino acids Molecular mass: 20159 Da Protein existence level: PE1 Quaternary structure: Heteromer composed of three CRYAA and one CRYAB subunits (PubMed:20836128). Aggregates with homologous proteins, including the small heat shock protein HSPB1, to form large heteromeric complexes (PubMed:10751411). Inter-subunit … WebDec 1, 2024 · Notably, the adenovirus E1b promoter, the zebrafish lepb 0.8-kb (P0.8) and lepb 2-kb (P2) promoters, and a new zebrafish synthetic promoter (ZSP) that combines elements of the E1b and P0.8 promoters drove little or no ectopic expression, making them suitable for transgenic assays. We also found significant differences in compatibility … newberg oregon burn information
Epigenetic Regulation of αA-crystallin in High Myopia-Induced …
WebSV40 polyA, zebrafish cryaa promoter (Kurita et al., 2003), AmCyan, SV40 polyA. For the myl7:Cre, cryaa:YPetulm13Tg line (abbreviated myl7:Cre), the following elements were assembled by PCR-based cloning techniques in a vector containing MiniTol2 repeats for transgenesis: 5.1 kb fragment of the zebrafish myl7 promoter, Cre recombinase WebApr 1, 2016 · The cryaa promoter drives high levels of expression in the lens of larval and adult zebrafish; when driving a fluorescent protein, it serves three purposes. First, it allows for the assessment of the efficiency with which the BAC transgene was injected into embryos based on the proportion of embryos with mosaic fluorescent protein expression … WebMar 17, 2016 · The CRYAA promoter with the rs7278468_T or rs7278468_G was transfected into HLE cells along with si-NC or si-KLF10, and the luciferase activity was measured 48 hours after transfection (the ... newberg or weather forecast