Cspb promoter

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August undefined, 2024

WebApr 18, 2024 · Construction of gadB2 expression strains under different RBS sequence and promoter. The nucleotide sequences of all primers are listed in Table 2.A series of RBS sequence derived from the consensus sequence AGGAG and an AS of 6–8 nt was designed and applied for expressing gadB2.For expressing with normal RBS sequence and AS of … WebThe xfp gene encoding PKT was cloned from Bifidobacterium animalis and overexpressed under the strong cspB promoter in C. glutamicum. A functional enzyme was detected in an L-Glu-producing strain of C. glutamicum (odhA). When cells of this producer strain with the xfp gene and those without the xfp gene were cultivated in a controlled ...

Promoters of Corynebacterium glutamicum - ScienceDirect

WebApr 27, 2011 · Therefore, vector pCRD314 containing tac promoter, SD sequence of tpi, and gfp gene was constructed. Second, tac promoter was replaced with promoters of PS1-encoding cspA or PS2-encoding cspB of C. glutamicum, as enhanced heterologous protein secretion was previously achieved using cspB promoter and cspA signal sequences … WebCPB distributes community service grants (CSGs) to noncommercial public television and radio stations that provide significant public service programming to their communities. … cisplatin used for

Enhanced production of recombinant proteins in

WebApr 26, 2011 · The promoter of the cspB gene coding for PS2 was found to direct expression of the Mycobacterium tuberculosis gene coding for the fibronectin-binding protein 85A (Salim et al. 1997), the Streptoverticillium … WebJan 16, 2024 · The Duffer Brothers, Brittany Howard, Lyle Lovett, Qui Nguyen, Angie Thomas, Billy Bob Thornton, Mary Steenburgen and Jesmyn Ward Among Featured … WebDec 1, 2011 · DNA fragments containing the promoter sequence of CspB from C. glutamicum and the signal sequence of CspA from C. ammoniagenes were fused to the CspB50TEV-Teriparatide gene. This synthesized construct was then inserted into the pPK4 plasmid and incorporated into the YDK010 bacterial strain, which is a derivative of wild … diamond\u0027s cafe madison

Ribosomal binding site sequences and promoters for

WebSep 1, 2006 · A biosynthetic pathway for poly(3-hydroxybutyrate) [P(3HB)] production by Corynebacterium glutamicum was developed by introducing the phbCAB operon derived from Ralstonia eutropha.P(3HB) synthase activity was detected in this recombinant C. glutamicum carrying a cell surface protein gene promoter. Intracellular P(3HB) was … WebMay 26, 2024 · The PΙNP gene with the cspB signal peptide was amplified and ligated to the above expression vectors. The PΙNP ... of l-lysine from raw corn starch by … cisplatin und gemcitabinWebNov 1, 1992 · The DNA sequence of the cspB promoter region is shown at the bottom. Interruption of the chromosomal copy of the cspB gene by a double-crossover recombination event. diamond\u0027s cy

"WebDec 31, 2002 · AmyA expression under the control of cspB promoter was assumed to be induced when raw starch was used as the sole carbon source. These results indicate that efficient simultaneous saccharification and fermentation of raw corn starch to L-lysine were achieved by C. glutamicum secreting AmyA using the cspB promoter and signal … " - Cspb promoter

Cspb promoter

WebDec 1, 2011 · Cell surface protein B (CspB) from Corynebacterium glutamicum is used as a pH-responsive peptide tag to enable a simple solid–liquid separation method for isolating … WebSep 4, 2003 · Synthesis of CspB is high when these strains are grown on lactate, acetate, gluconate and tartrate but low when the carbon sources are glucose, fructose, sucrose …

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WebApr 1, 2006 · Furthermore, the promoter of the cspB gene was mapped by 5′ rapid amplification of cDNA ends PCR and the corresponding DNA fragment was used in DNA affinity purification assays. A 30 kDa protein specifically binding to the promoter region of the cspB gene was purified. Matrix-assisted laser desorption ionization time-of-flight … WebThe synthetic promoter library covered a wide range of strengths, ranging from 1 to 193% of the tac promoter. 68 promoters were selected and sequenced for correlation analysis between promoter ...

WebJan 31, 2015 · In the present study, we show that cspB, cspG and cspI, the members of cspA family and induced in response to cold shock (reviewed by Gualerzi et al. 2003), … WebJul 3, 2024 · The promoter of elongation factor Tu (tuf), which is known as a strong promoter in C. glutamicum, was amplified by PCR using primers P15 and P16 for the …

WebThe coding and promoter regions of the cspB gene encoding PS2 were cloned in lambda gt11 using polyclonal antibodies raised against PS2 for screening. Expression of the … WebNov 1, 1992 · The DNA sequence of the cspB promoter region is shown at the bottom. Interruption of the chromosomal copy of the cspB gene by a double-crossover …

WebJul 5, 2016 · A total of 84 predicted promoter sequences located upstream of different classes of proteins including heat shock proteins, cell-envelope proteins, and proteins resistant against toxic metals (based on similarity) …

WebA new class of cold shock-induced proteins that may be involved in an adaptive process required for cell viability at low temperatures or may function as antifreeze proteins in … diamond\\u0027s celebration of lifeWebunder the control of the cspB promoter were constructed and introduced into the C. glutamicum Cm strain. Immu-nostaining microscopy and flow cytometric analysis revealed that PorB-AmyA, PorC-AmyA, and PorH-AmyA were displayed on the C. glutamicum cell surface. AmyA activity was only detected in the cell fraction of C. diamond\\u0027s community based servicesWebJan 1, 1999 · In contrast to cspB and cspC, whose promoters and 5 UTRs share a high degree of sequence identity, cspD lacks a long 5 UTR and has a dissimilar putative promoter sequence (Graumann et al. 1997). The presence or absence of the long 5 UTR may explain the differential stationaryphase induction of B. subtilis CSPs. diamond\u0027s chWebcspB promoter was constructed by cross-over PCR as described previously (Kikuchi et al. 2006). First-round PCR amplified the cspB promoter using Primer A (Csp5K, forward primer, Table 2) and Primer B (Csp-PPPg, reverse primer) and pPSPTG11 DNA as a template; the pro-PG gene with its own signal sequence was also amplified using diamond\u0027s cwWebDownload scientific diagram Genetic features of the cspB promoter region of C. glutamicum ATCC 14067 and visualization by SDS-PAGE of proteins binding in front of the cspB gene. (a) The ... diamond\\u0027s cause of deathWebFeb 6, 2012 · The latter is flanked by two loxP sites and constitutes of a transcriptional promoter (P-35S), the nptII coding sequence (CS-nptII), and a polyadenylation sequence from the nopaline synthase gene (T-nos). MON 87460 contains one copy of the insert at a single insertion site hosting both cspB and nptII intact expression cassettes. diamond\u0027s cause of deathWebThe major cold shock protein of Bacillus subtilis CspB binds with high affinity to the ATTGG- and CCAAT sequences in single stranded oligonucleotides ... a cis-element found in the promoter region of mammalian major histocompatability complex class II genes, which has the consensus sequence CTGATTGGC/TC/TAA [4,5]. The underlined penta- cisplatin vision changes