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Phospho explorer antibody array

WebThe Human Phospho-Kinase Array (Catalog # ARY003B) is a rapid, sensitive, and economical tool to simultaneously detect the relative site-specific phosphorylation of 43 kinases and 2 related total proteins without performing numerous immunoprecipitations and Western blots. WebFeb 28, 2024 · Building antibody arrays using capture reagents targeting proteins in the signalling cascade of interest has enabled researchers to investigate changes in protein profiles and modifications in signalling pathways in …

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WebOct 27, 2024 · Phospho Explorer antibody array experiments and analyses were performed as a custom service by E-Biogen (Ebiogen Inc., Seoul, Republic of Korea). Overall design HUVECs were serum-starved for 8 hours and then incubated with an anti-TGFβ1 antibody for 1 hour, after which HUVECs were stimulated with 1μg/ml DG-LRG1 for 30 minutes. WebMar 25, 2016 · Phospho-protein profiling by Phospho Explorer Antibody Array Cell lysates obtained from PC-1 and PC-1.0 cells were applied to a Phospho Explorer Antibody Array (Full Moon Biosystems, Sunnyvale, CA, USA). The array contained 1318 antibodies. iphone make picture size smaller https://chriscrawfordrocks.com

Phosphoproteome profiling provides insight into the mechanism …

WebOne of the best selling antibody arrays with 269 key phospho-specific and site-specific antibodies from multiple cancer signaling pathways. Cytokine Profiling Array Featuring 310 cytokine related antibodies. Each antibody is printed with six replicates. Ideal for cytokine profiling and inflammation studies. Phospho Explorer Array WebApoptosis Phospho Antibody Array This array is designed for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. This array features site-specific and phospho-specific antibodies, allowing researchers to study serine/threonine and tyrosine phosphorylation at specific sites. WebPhospho Explorer Antibody Array is a glass-based antibody array for broad-scope protein phosphorylation profiling and screening in cell and tissue samples from human, mouse and rat. This array consists of 1318 antibodies related to multiple signaling pathways and … Antibody Array Assay Kit is designed for easy and reliable processing of antibody … Chen P, Huang H, Bone marrow stromal cells protect acute myeloid leukemia … Signaling Explorer Antibody Array features 1358 unique antibodies covering 20 cell … Signaling Explorer Array: Garnett D, Caveolae as a target to quench … iphone makes loud noises with earbuds

Example Protocol for Protein Arrays - BioCat

Category:Proteome Profiler Human Phospho-Kinase Array Kit …

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Phospho explorer antibody array

Phosphoproteome profiling provides insight into the mechanism …

WebApr 13, 2024 · We used the Phospho Explorer Antibody Array, which encompasses nearly all known signaling pathways, to assess molecular mechanisms underlying LPHN2-induced cardiac differentiation. LPHN2-dependent phosphorylation was the strongest for cyclin-dependent kinase 5 (CDK5) at Tyr15. WebPhospho Explorer Antibody Array data of KGN cells treated with Hu‐17. (A) Heatmap of proteins differently phosphorylated proteins in KGN cells with Hu‐17 treatment. The data included in the heatmap is beyond cutoff line with the change fold is ≤77% or ≥130%. A is the control group, B is the Hu‐17 treated for 3 h group, C is the Hu ...

Phospho explorer antibody array

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WebImmunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190). Immunofluorescence; Immunofluorescence staining of methanol-fixed HeLa cells using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190, red). WebMoon BioSystems’ antibody arrays allow researchers to detect and analyze hundreds of proteins simultaneously on a single slide, saving precious resources and reducing the number of variables that affect experimental outcome. Our unique collection of antibody arrays includes phospho-specific arrays

Web46 rows · The Proteome Profiler Human Phospho-Kinase Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Phosphorylation of captured proteins is detected with biotinylated phospho-specific detection antibodies … WebApr 29, 2024 · Phosphoprotein profiling by the Phospho Explorer Antibody Array PEX100, which was designed and manufactured by Full Moon Biosystems, Inc. (Sunnyvale, CA), contains 1318 antibodies. Each of the antibodies has two replicates that are printed on coated glass microscope slide, along with multiple positive and negative controls.

WebHuman Protein S-Nitrosylation Array G2 The RayBiotech G-Series Human Protein S-Nitrosylation Antibody Array 2 is a multiplex assay on glass slide for the relative detection of cystein s-nitrosylation of 493 different human proteins cell lysate, culture supernatant, serum, plasma and other biological samples. WebCompared to the conventional methods, our antibody arrays provide a much more cost-effective means for protein research. Investigators can choose from over 40 different arrays to quickly compare protein expression or …

WebSep 1, 2024 · Total RNA was extracted by chloroform and isopropanol precipitation. cDNA was obtained using the SuperScript III First-Strand Synthesis System (Invitrogen, 18080-051). qPCR analyses were performed with primers listed below using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, 1725270) in at least duplicate in three …

WebNov 11, 2024 · First, we used a previously described intrabody30 (Ib30)-based sensor for βarr1, which selectively recognizes receptor-bound conformation of βarr1 and reports agonist-induced formation of the receptor-βarr1 complex in cellular context ( Baidya et al., 2024a; Baidya et al., 2024b ). iphone makes noise when home button hitsWebWe used the Phospho Explorer Antibody Array, which encompasses nearly all known signaling pathways, to assess molecular mechanisms underlying LPHN2-induced cardiac differentiation. LPHN2-dependent phosphorylation was the strongest for cyclin-dependent kinase 5 (CDK5) at Tyr15. We identified CDK5, Src, and P38MAPK as key downstream … orange co nc gis mapWebWhat Array format do you want? All Arrays. 96-well standard 96-well Fast. 384-well. OpenArray. What type of primers are you looking for? PCR/Sanger Sequencing Primers) Enter target information. Enter / Upload Multiple Targets. Enter Single Sequence. Select a single species to search by sequence. What chromosome position are you interested in? ... orange co homes for saleWebAug 3, 2024 · A phospho-protein array analysis revealed that PF-3758309 down-regulates the NF-κB signaling pathway, which provides the most likely mechanism by which PF-3758309 inhibits latency reversal. orange co health dept orlando flWebThe antibody array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison. Learn more about cytokine … iphone makes siren soundWebMay 13, 2024 · Phosphodiesterase type 5 (PDE5) inhibitors are the most commonly used first-line treatment options for ED; however, these agents are ineffective in ~30% of patients and ultimately cannot rescue... orange co library vaWebAKT Phospho-Specific Array includes 137 highly specific and well-characterized phosphorylation antibodies in the AKT pathway. Product detail AKT/PKB Signaling Phospho-Specific Array orange co nc health dept