Phosphotyrosine 4g10
WebCells were lysed and underwent immunoprecipitation (IP) with antibody specific for Jak2 or Stat5; Western blot (WB) was then performed with antibodies against phosphotyrosine (4G10), total Jak2 ... WebSep 19, 2016 · These data suggest that the Src superbinder may provide an alternative means to commonly used anti-phosphotyrosine antibodies 13 such as 4G10 and P-Tyr-100 for the purification of...
Phosphotyrosine 4g10
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WebSep 8, 2015 · Here we compared two commercially available phosphotyrosine antibodies and show that P-Tyr-1000 yields 64% more phosphopeptides than 4G10 antibody, while including almost all 4G10 captured phosphopeptides. The workflow can be reproducibly performed at intermediate protein input levels of 10mg. WebChemical/ Small Molecule corresponding to Phosphotyrosine conjugated to keyhole limpet haemocyanin. Positive control ICC/IF: C2C12 cells treated with 2mM H2O2 for 10min. WB: NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate. General notes This antibody clone is manufactured by Abcam.
WebSen Chen's publication in Sci Signal. ...against pTyr (4G10) and tubulin were from Millipore, and antibody against pMET (Tyr1234) was from GeneTex. Bmxtides were from … WebAntibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
WebJun 15, 2012 · Phosphopeptide chip technology and antibody profile. Peptide chips containing three identical arrays of 6400 phosphopeptide spots were incubated with the indicated anti-phosphotyrosine antibodies (4G10, p-TYR-100 and pY20) and a fluorescently labelled secondary antibody. Web11 rows · 4G10 Platinum, Anti-Phosphotyrosine (mouse monoclonal …
WebPolyclonal Abs specific for phosphorylated forms of eNOS, p38, JNK, ERK1/2 (eNOS Ser1177, Erk1/2 Thr202/Tyr204, p38 Thr180/Tyr182 and SAPK/JNK Thr183/Tyr185) were from Cell Signaling Technology (Beverly, MA, USA); the anti-phosphotyrosine (4G10) was from Upstate Biotechnology, Millipore (Billerica, MA, USA), and the monoclonal antibody …
WebApr 1, 2010 · Tyrosine phosphate is a frequently observed modification in a limited number of protein molecules, principally the products of viral oncogenes and membrane receptors for mitogenic growth factors.... list us government agencieshttp://www.cnreagent.com/s/sv119268.html impact world titleWebAnti-phosphotyrosine Antibody, clone 4G10 ZooMAb® Mouse Monoclonal 市场价 ￥4,945.45 好评率 100% (0)条评论 商城价 登录后可查看价格 list usual causes of cystitis and urethritisWebJan 12, 2012 · Embryos were incubated with biotin-conjugated phosphotyrosine (anti-4G10, Millipore) antibody overnight at 4°C at a dilution of 1 : 500 (in block solution). Embryos were then washed (3 × 10 min) in PBDT and incubated for 2 hours at room temperature with streptavidin conjugated Cy3 or QDs 655 nm at 1 : 500 dilution in fresh block solution. impact world tourWebJun 15, 2012 · The 4G10, pY20 and p-TYR-100 antibody specificity: profiling by peptide microarrays The reversible phosphorylation of tyrosine residues is one of the most … impact world history textbookWebAnti-Phosphotyrosine Antibody, clone 4G10® Anti-Phosphotyrosine Antibody, clone 4G10 detects tyrosine phosphorylated proteins in all species. This unique monoclonal antibody … impact worldwideWebAnti-Phosphotyrosine Antibody, clone 4G10®, HRP conjugate: Overview: Anti-Phosphotyrosine antibody (05-321), cross-linked to horseradish peroxidase: Background Information: Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as … impact world title belt