WebpSecTag2 and pSecTag2/Hygro are mammalian expression vectors designed for the secretion, purification, and detection of fusion proteins. Each vector has a large multiple … WebpSecTag2 A Mammalian expression vector for the secretion of C-terminally 6xHis-tagged proteins. For other reading frames, use pSecTag2 B or pSecTag2 C. Sequence Author: …

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WebNov 1, 2006 · These sequences were selected for DNA vaccine design based on pSecTag2-A (pSA) also including a T-cell helper epitope. GD2 mimicry was shown following transfection of CHO-1 cells with pSA-MA and pSA-MD DNA vaccines, with twice-higher signal intensity for cells expressing MD over MA. WebYou could try to eluate your plasmid using hot water, at 65-70°C. It help the release of the plasmid from the column. Cite. 1 Recommendation. 1st Oct, 2024. Abir Chakraborty. Rhodes University. I ... pink stone birthstone

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WebA, shows the pSecTag2/ HygroB mammalian expression vector construction. Human proBDNF was subcloned into the expression vector after murine Ig-Kappa chain V-J2-C … WebUse pSecTag2 A for functional genomics research and drug discovery study. 2024-nCoV Research Solution Guide; Monkeypox Virus Real Time PCR Kit; Monkeypox Pseudovirus; … WebDec 1, 2006 · The schematic illustration of the eukaryotic expression vector pSecTag2/IL-2-183B2scFv was presented as Fig. 1 A. 183B2scFv (753 bp) was PCR amplified using primers that added restriction endonuclease recognition site ApaI to the 3′-end and XhoI to the 5′-end. The PCR fragment was ligated into pSecTag2 for transformation into competent … steffan evans comedian