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WebDec 23, 2024 · RNA quality was examined on 1% Agarose gels, and the purity and concentration were analyzed using a Nano-Photometer spectrophotometer (IMPLEN, CA, United States). cDNA synthesis for gene cloning and qRT-PCR was performed with a 10 μg total RNA pool produced by mixing equal volumes of the three RNA replicates in a tube … WebJun 1, 2024 · They went into business, initiating attempts to clone Aiken Cura at ViaGen, a laboratory in Meeker’s native Texas that offers pet and livestock cloning services. A year … WebFeb 6, 2024 · In random (non-directional) cloning, double-stranded cDNA are ligated directly to a blunt-end cloning vector. In directional cloning, adapters with rare restriction sites … denison women\u0027s basketball schedule