Webb1 dec. 2024 · Newer SHAPE reagents have demonstrated in vivo reactivity within multiple organisms, although use of these reagents requires consideration of their different chemical properties [54 •]. All SHAPE reagents, which acylate the 2’-O, also react with water; this hydrolysis quenches the reagent but also limits its intracellular half-life. WebbIn addition to different SHAPE reagents, two SHAPE assays, icSHAPE (in vivo click selective 2’-hydroxyl acylation and profiling experiment) [17] and SHAPE-MaP [18] have …
Direct detection of RNA modifications and structure using single ...
http://yang-laboratory.com/static/publication_pdf/2024_Methods_SHAPE-MaP.pdf Webb31 aug. 2024 · For the synthesis of SHAPE reagents, 2-methylpyridine-3-carboxylic acid 10(cat. 325228), isoquinoline-6-carboxylic acid (cat. APO455831358-250MG), indoline- … bis wotlk prot warrior
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WebbDescription. In Vivo SHAPE Reagent is used for the determination of RNA structure. This reagent contains a highly pure form of 2-methylnicotinic acid imidazolide (NAI). This … Webb26 jan. 2024 · Selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) chemical probing provides local RNA flexibility information at single-nucleotide … WebbDescription. In Vivo SHAPE Reagent is used for the determination of RNA structure. This reagent contains a highly pure form of 2-methylnicotinic acid imidazolide (NAI). This compound forms 2’-O-adducts on single stranded regions of RNA that can be detected by Selective 2’-Hydroxyl Acylation and Primer Extension (SHAPE). bis world of warcraft