Tthssb
http://www.100md.com/html/DirDu/2006/07/27/12/18/65.htm WebAug 10, 2006 · These results were surprising in the context of half-life of SSB from thermophilic T. aquaticus, which has only 30 s of half-life at 95 degrees C. DrpSSB is the …
Tthssb
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WebThermus thermophilus (TthSSB). The three proteins were found as dimers in solution. They bound in vitro to single-stranded DNA specifically over a temperature range of 4–80oC, and the wild-type protein could withstand incubation at 94oC for 2 min. Addition of TthSSB to PCR halved the elonga-tion time required for the DNA polymerases of WebHere we show, using single-molecule fluorescence resonance energy transfer (FRET), that homodimeric bacterial SSB from Thermus thermophilus (Tth) is able to diffuse …
WebFurthermore, TthSSB increases the fidelity of proofreading deficient Thermus thermophilus DNA polymerase (Perales et al., 2003). We tested the slippage properties of PabPolB, ... Webdate, offering an attractive alternative to TaqSSB and TthSSB in molecular biology applications, especially with using high temperature e. g. polymerase chain reaction (PCR). Background Single-stranded DNA-binding (SSB) proteins play an essential role in allin vivo processes involving ssDNA. They interact with ssDNA and RNA, in an independent
WebA fixed quantity (20 pmol) of 5h-end fluoresceinlabelled oligonucleotide (dT) 30 was incubated with 20 pmol of TaqSSB or TthSSB (#) in 20 µl reaction mixtures in standard … Webfrom T.thermophilus strain HB8 (TthSSB) increases the effi-*To whom correspondence should be addressed. Tel: +81 45 508 7224; Fax: +81 45 508 7364; Email: [email protected] The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors The Author 2006. Published by Oxford University Press.
WebAug 2, 2006 · These results were surprising in the context of half-life of SSB from thermophilic T. aquaticus, which has only 30 s of half-life at 95°C. DrpSSB is the most thermostable SSB-like protein identified to date, offering an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and analytical purposes.
WebHere we show, using single-molecule fluorescence resonance energy transfer (FRET), that homodimeric bacterial SSB from Thermus thermophilus (Tth) is able to diffuse spontaneously along ssDNA over a wide range of salt concentrations (20-500mM NaCl), and that TthSSB diffusion can help transiently melt the DNA hairpin structures. fluss in florenzhttp://www.diva-portal.org/smash/record.jsf?pid=diva2:658627 fluss in nordportugal 3 buchstabenWebAfter binding single-stranded DNA, SSB destabilizes helical duplexes, thereby allowing DNA polymerases to access their substrate more easily. SSB is involved in DNA replication and … green glass bubble cabinet knobsWebNov 15, 2003 · The thermostability of TthSSB was also studied. To this end, TthSSB was pre‐incubated at 94°C for different times, cooled down to 70°C, and immediately … fluss in gothaWebssDNA Binding Proteins. Product Listing Product Overview. NEB offers highly thermostable ssDNA binding proteins that are ideal for nucleic acid amplification and sequencing (Tth … green glass bottle with cork stopper ball lidWebJul 27, 2006 · TthSSB-255 could only enhance RCA reactions drastically, which showed weak ssDNA binding affinity. This characteristic of the protein would be critical for reduction of nonspecific products. As shown in Supplementary Figure SP2, TthSSB has a significant cluster consisting of acidic residues in its C-terminus although the role has not been … fluss in lyonWebAug 2, 2006 · These results were surprising in the context of half-life of SSB from thermophilic T. aquaticus, which has only 30 s of half-life at 95 degrees C. DrpSSB is the most thermostable SSB-like protein identified to date, offering an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and analytical … fluss in ostengland